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goat polyclonal anti 179 hbegf  (R&D Systems)


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    Structured Review

    R&D Systems goat polyclonal anti 179 hbegf
    Goat Polyclonal Anti 179 Hbegf, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 307 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/polyclonal+goat+anti+human+hb+egf+antibody/Human+HB-EGF+Antibody/pm40562080-75-0-5
    Average 95 stars, based on 307 article reviews
    goat polyclonal anti 179 hbegf - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Incubation:

    Article Title: An EGFR autocrine loop encodes a slow-reacting but dominant mode of mechanotransduction in a polarized epithelium
    Article Snippet: .. Briefly, 96-well flat-bottomed plates (Nunc MaxiSorp; Nunc, Rochester, NY, USA) were incubated with polyclonal goat anti-human HB-EGF antibody (1 μg/ml; R&D Systems) at room temperature for 8 h. Plates were then blocked overnight at 4°C with blocking buffer [Tris-buffered saline (TBS) plus 3.5% BSA]. .. After washing with TBS plus 0.05% Tween (TBST), recombinant protein standards (R&D Systems) and medium samples were added to wells and incubated at 37°C for 2 h. Following repeated washing with TBST, biotinylated antibody (0.5 μg/ml polyclonal goat anti-human; R&D Systems) against the relevant ligand was added to each well for 1.5 h at room temperature.

    Blocking Assay:

    Article Title: An EGFR autocrine loop encodes a slow-reacting but dominant mode of mechanotransduction in a polarized epithelium
    Article Snippet: .. Briefly, 96-well flat-bottomed plates (Nunc MaxiSorp; Nunc, Rochester, NY, USA) were incubated with polyclonal goat anti-human HB-EGF antibody (1 μg/ml; R&D Systems) at room temperature for 8 h. Plates were then blocked overnight at 4°C with blocking buffer [Tris-buffered saline (TBS) plus 3.5% BSA]. .. After washing with TBS plus 0.05% Tween (TBST), recombinant protein standards (R&D Systems) and medium samples were added to wells and incubated at 37°C for 2 h. Following repeated washing with TBST, biotinylated antibody (0.5 μg/ml polyclonal goat anti-human; R&D Systems) against the relevant ligand was added to each well for 1.5 h at room temperature.

    Saline:

    Article Title: An EGFR autocrine loop encodes a slow-reacting but dominant mode of mechanotransduction in a polarized epithelium
    Article Snippet: .. Briefly, 96-well flat-bottomed plates (Nunc MaxiSorp; Nunc, Rochester, NY, USA) were incubated with polyclonal goat anti-human HB-EGF antibody (1 μg/ml; R&D Systems) at room temperature for 8 h. Plates were then blocked overnight at 4°C with blocking buffer [Tris-buffered saline (TBS) plus 3.5% BSA]. .. After washing with TBS plus 0.05% Tween (TBST), recombinant protein standards (R&D Systems) and medium samples were added to wells and incubated at 37°C for 2 h. Following repeated washing with TBST, biotinylated antibody (0.5 μg/ml polyclonal goat anti-human; R&D Systems) against the relevant ligand was added to each well for 1.5 h at room temperature.

    Recombinant:

    Article Title: Crosstalk between purinergic receptor P2Y 11 and chemokine receptor CXCR7 is regulated by CXCR4 in human macrophages
    Article Snippet: The ATP analog ATPγS (Sigma Aldrich, St. Louis, MO, USA) and the suramin analog NF340 (Santa Cruz, Dallas, TX, USA) were utilized as P2Y 11 receptor agonist (20 μM) and antagonist (20 μM), respectively. .. Additional reagents employed in this study: the PDE4-selective inhibitor rolipram (10 μM) (Sigma-Aldrich), recombinant IL-1β (0.2 and 2 ng·ml − 1 ) (R&D Systems, Minneapolis, MN, USA), the selective EGFR tyrosine kinase inhibitors AG1478 (10 μM) (Sigma) and erlotinib (20 μM) (Cayman Chemical, Ann Arbor, MI, USA), the CXCR4 chemokine receptor antagonists mavorixafor (AMD 070; 2–10 μM), plerixafor (AMD 3100; 2–10 μM), which is also an allosteric agonist of CXCR7, and TC14012 (2–10 μM), which is also a potent CXCR7 agonist (all from MedChemExpress, Monmouth Junction, NJ, USA), the MEK/ERK inhibitor U0126 (10 μM) (Sigma), the ACKR3 (CXCR7) chemokine receptor agonist VUF11207 (100 nM) (MedChemExpress), the TACE/ADAM17 inhibitor TAPI-1 (20 μM) and the ADAM10 inhibitor GI 254,023 × (10 μM) (both from Tocris, Bristol, UK), a polyclonal goat anti-human HB-EGF antibody (10 μg·ml − 1 ) (AF-259-SP; R&D Systems, Minneapolis, MN, USA), a polyclonal goat anti-human TGFα antibody (10 μg·ml − 1 ) (AB-239-NA; R&D Systems), a polyclonal goat anti-human CXCL12 antibody (10 μg·ml − 1 ) (PA1-20154; Invitrogen / Thermo Fisher Scientific, Waltham, MA, USA), and a monoclonal mouse anti-human CXCL11/I-TAC antibody (10 μg·ml − 1 ) (MAB672; R&D Systems). ..



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